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cardosopedro

Member Since 02 Jun 2009
Offline Last Active May 14 2013 04:52 AM

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My research interests
Programmed cell death

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Posts I've Made

In Topic: Identifying new Ca2+-binding proteins

14 May 2013 - 04:55 AM

I guess I'll try it and see what happens.

In Topic: Identifying new Ca2+-binding proteins

14 May 2013 - 02:16 AM

I was reading the paper you sent me and also other literature about this stains-all reagent and I although I'm leaning to try it, I was wondering about this: the idea is to compare stains all-stained SDS-PAGE gels loaded with protein extracts from the wild type vs the knockouts for my candidate proteins, right? However, since each extract contains thousands of proteins, that is, lots of bands in the gel, I was wondering if my eye will be sensitive enough to detect the lack of just one band in the lanes with the extracts from the knockouts...

Apparently, from what read, people normally use stains-all to stain purified protein rather than extracts. In that paper you recommended they use extracts but from sarcoplasmic reticulum vesicles which means they have a restricted number of proteins in the gel...

Please give me your opinions.

Thanks!

In Topic: Identifying new Ca2+-binding proteins

13 May 2013 - 06:40 AM

Thank you. I'll definitively try that.

In Topic: Identifying new Ca2+-binding proteins

10 May 2013 - 04:08 AM

The genome of the organism I work with is fully sequenced so I already have the sequence of these proteins. I predicted the presence of Ca2+-binding motifs using this tool (http://gallium.gsu.edu:8080/seq_f/) but I would to prove it biologically. At least indirectly, since I don't intend to produce the recombinant proteins.

In Topic: NADPH monitoring

23 April 2013 - 11:23 AM

Thanks.

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