1. One of your target gene has feedback regulation
2. The primer sets you used for genome-typing are not good for RT-qPCR or qRT-PCR especially when you have genomic DNA contamination.
3. Dosage issue or shRNA potency; the same dosage works for your colleague because his target gene can be silenced in a lower concentration of shRNA. You might want to screen more potent shRNA by playing around the ratio of shRNA vector/luc-target vector (e.g. 1:1 or 1:5). Then use the shRNA for your in vivo experiments.
ravibiosciencesMember Since 06 May 2009
Offline Last Active Mar 30 2013 09:41 AM
PhD Student from the University of Heidelberg working in the areas of Vascular endothelial cell signaling.
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- Age 30 years old
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My research interests
Molecular cell biology of human endothelial cells