Find content
Male
1. One of your target gene has feedback regulation
2. The primer sets you used for genome-typing are not good for RT-qPCR or qRT-PCR especially when you have genomic DNA contamination.
3. Dosage issue or shRNA potency; the same dosage works for your colleague because his target gene can be silenced in a lower concentration of shRNA. You might want to screen more potent shRNA by playing around the ratio of shRNA vector/luc-target vector (e.g. 1:1 or 1:5). Then use the shRNA for your in vivo experiments.
#82731 shRNAs work in vitro, not in vivo
Posted
on 07 August 2010 - 12:24 PM
