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In Topic: Inhibition of a protein upstream in signaling cascade ?
25 March 2013 - 01:12 AM
As you said you have very short time. I mean to say, it might take long time than you expected to get it published
In Topic: Inhibition of a protein upstream in signaling cascade ?
24 March 2013 - 04:12 AM
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Y
To know the best possible signalling mech in any pathway is to use a) Pharmacological inhibition or
. RNA interference. You have no drug which inhibits your X and you don't want to work with siRNA exp's either.
Two questions i would expect and look for a convincing answer.
1. I guess you might have already considered over expression of your X to find out or rule out the functional effects leading to your hypothesis via "Y"? (of-course, with proper +/- controls)
2. Is there any stimulant that you can show direct regulation of your ''Y'' irrespective of your upstream targets?
If you want to play with upstream targets, I am afraid to get increased impact of your paper in a very short time
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Good luck and enjoy doing science.
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Y
To know the best possible signalling mech in any pathway is to use a) Pharmacological inhibition or
. RNA interference. You have no drug which inhibits your X and you don't want to work with siRNA exp's either.Two questions i would expect and look for a convincing answer.
1. I guess you might have already considered over expression of your X to find out or rule out the functional effects leading to your hypothesis via "Y"? (of-course, with proper +/- controls)
2. Is there any stimulant that you can show direct regulation of your ''Y'' irrespective of your upstream targets?
If you want to play with upstream targets, I am afraid to get increased impact of your paper in a very short time
.Good luck and enjoy doing science.
