lyok

Hallo all,

I have a problem with NCBI.

I have a sequence and I want to know what it might be.

Now what I do is the following:
I go to the NCBI website and then I blast this.
When I do this, I get a page with some results (see picture 1)
I see a lot of names with results.

But whats next?


What I do, is check the best result, look for the position of that gene.

Eg: here it states that the gene starts at position 24.409 (see picture 2)
But how do I find that gene without the need to manually start looking for it on the page that has the sequence of the bacterium with the sequence I am looking for? Or is this the only way to do it?... so do I need to manually look up position 24.409 in the genome of that bacterium to fine my gene?(see picture 3)? Or is there an easier method?

I hope my question is a bit clear?
 fotke1.jpg (192.88K)
Number of downloads: 19  fotke2.jpg (207.69K)
Number of downloads: 23  fotke3.jpg (153.9K)
Number of downloads: 13


Another question: what if you have more then 1 99% similarity result? Here I have more... I cant look them all up manually? This will take days?

+ I did it with 1 gene , checked 2 results and both results give me another gene (1 says its a phage DNA package gene and 1 says its a bacerium gene).

Hallo all,

I have the following problem with 454: when the samples are prepared (adaptors put on the samples, made the DNA single stranded), the samples are then transferred to the beads.
Now my question is: if 1 DNA strand attaches to the bead, then what happens?

==> this DNA strand is bound to the bead and amplified, now after this step, what happens? The strand that you amplified breaks lose and gets bound at anohther place on the bead? Or is it the original strand (the one from your sample) that breaks lose and attaches at another place?
And how do you make sure the right strand breaks lose? Because you want all identical strans on your bead in the end.. you need to make sure that its the correct strand that breaks lose everytime, how do you do this?

Hallo all,

Has anyone an idea how to know whether certain patents are reffered to in scientific journals?
I have been looking up some intersting patents, but I wonder if they have been used/cited by other researchers.
Is there an easy way to find this kind of information? At the moment, I try to google it, but its hard to find some information about it.
Also: because there is no registered trade name, its hard to look it up by a simple name.

any ideas?

thanks

Hallo,

I have been given the following task: you have cockroaches and in these cockroaches you will find unicellular eukaryotic cells that are visable with the naked eye, how would you isolate these cells and grow then?

This is what I came up with:

since they are visable with the naked eye, I would dissect some of the cockroaches, look for the unicellular cells and simple collect them in fresh media for eukaryotic cells and check if they grow.
But where I am stuck is: how do I make sure I only have the correct cells and not for example bacteria?
I would think, I could add some antibotics to the fresh media to have a pure culture?


And then if I have them isolated, I would need to check if they are doing allright, maybe I can do some viability test?

Any remarks?

Hallo,
I havent had a lot of courses in biochemistry/enzymekinetics and I am struggling with a few things about enzymes.

For example on the wikipedia article about enzymes they mentions the following: "Enzymes catalyze the forward and backward reactions equally." What does this mean?
(link: http://en.wikipedia.org/wiki/Enzyme)

I checked it and I (seem) to understand the example (carbonic anhydrase, should catalyse both reactions), but does it mean that every enzyme catalyses both the forward and backward reactions? Or not all enzymes?

I am getting a bit confused because I learned that often: A => B (done by enzyme X) while B => A is done by another enzyme.

So does it just depend on the specific reaction?
Or?

And what with for example this: http://en.wikipedia....:Glycolysis.svg , you see some <=> in the shedule, does a <=> mean that both => and <= are catalysed by the same enzyme? Or does it mean that an enzyme can catalyse => and that <= can happen spontanously? (or maybe that both => and <= are catalysed by the same enzyme?)


Thanks in advance.