lyok

Hallo all,

I have a problem with NCBI.

I have a sequence and I want to know what it might be.

Now what I do is the following:
I go to the NCBI website and then I blast this.
When I do this, I get a page with some results (see picture 1)
I see a lot of names with results.

But whats next?


What I do, is check the best result, look for the position of that gene.

Eg: here it states that the gene starts at position 24.409 (see picture 2)
But how do I find that gene without the need to manually start looking for it on the page that has the sequence of the bacterium with the sequence I am looking for? Or is this the only way to do it?... so do I need to manually look up position 24.409  in the genome of that bacterium to fine my gene?(see picture 3)? Or is there an easier method?

I hope my question is a bit clear?



Another question: what if you have more then 1 99% similarity result? Here I have more... I cant look them all up manually? This will take days?

+ I did it with 1 gene , checked 2 results and both results give me another gene (1 says its a phage DNA package gene and 1 says its a bacerium gene).

K.B., on 14 April 2012 - 03:13 AM, said:

I do not see what it is wrong with that question.

Deionized water (demineralized water) is not bacteria free... Its not sterile water...
Same goes for dd water.
The changes to get bacteria in it are even higher since people use the same containers over and over and they are not sterile at all!
Even MQ water can contain bacteria.

I do not know from what country you are, but in my country, tap water is pretty clean.

So the question remains: why boil the water the tap water for the 70% ethanol solution and not, for example, boil the di water?

Also: if you boil the water: the dead bacteria will stay in it.. so you dont remove it, so dont see the point trof made since in di water there are bacteria present too.

PS. the standards for tap water (CFU) in many countries is more strict then those for purified water (often no regulation to CFUs at all)

There is most likely a reason why not to use tap water, but your explenation isnt 100% accurate.

So, not sure whether your *facepalm* should be repeated....


(I am strictly speaking of cleaing a bench, not speaking about using the water for other purposes, ddwater etc has its purpose, but not sure why you need to use it for a 70% ethanol solution.. It has to do with the non bacterial susbtances in the water I think, rather then the bacterial ones)