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Felipillo, on 23 May 2012 - 10:36 AM, said:

Ok, thanks for the links.

Why should I use the E-value in stead?
BTW: in my specific situation here, the e-value is the same for the 99% values (or at least for some).
And to be honest: I am not that inclined to check all the "high scoring" genes.. it would take me days.. I just checked 1 or 2 each time.



I am not sure what you mean with: "Also if you do not need to look for regulatory elements in DNA, it's easier to work with proteins, they have signature conservation and protein families, that help you to infer protein function for unknown sequences.".

How can I work with a protein if I just have a DNA sequence?
Can I simple translate the DNA sequence into a protein? But how can I do this, because I cant know in advance what part of the sequence is coding for the protein, right?

Trof, on 14 April 2012 - 07:14 AM, said:

MQ is indeed the most pure form of water, but I do not know a lot of labs where they prepare ethanol 70% with MQ.
Do you really make ethanol solutions with MQ? Very very very expensive.

Oh yeah, and I forget to tell: in the ion-exchange units used to make demineralized water, you often have growth of algaes... not that clean either...

K.B., on 14 April 2012 - 03:13 AM, said:

I do not see what it is wrong with that question.

Deionized water (demineralized water) is not bacteria free... Its not sterile water...
Same goes for dd water.
The changes to get bacteria in it are even higher since people use the same containers over and over and they are not sterile at all!
Even MQ water can contain bacteria.

I do not know from what country you are, but in my country, tap water is pretty clean.

So the question remains: why boil the water the tap water for the 70% ethanol solution and not, for example, boil the di water?

Also: if you boil the water: the dead bacteria will stay in it.. so you dont remove it, so dont see the point trof made since in di water there are bacteria present too.

PS. the standards for tap water (CFU) in many countries is more strict then those for purified water (often no regulation to CFUs at all)

There is most likely a reason why not to use tap water, but your explenation isnt 100% accurate.

So, not sure whether your *facepalm* should be repeated....


(I am strictly speaking of cleaing a bench, not speaking about using the water for other purposes, ddwater etc has its purpose, but not sure why you need to use it for a 70% ethanol solution.. It has to do with the non bacterial susbtances in the water I think, rather then the bacterial ones)

K.B., on 13 April 2012 - 11:56 PM, said:

Why should you boil the water?
Tap water should be pretty clean, so I imagine you wan to boil it to remove other componets?

And why should you, in the first place, use dd, DI or milliQ water? Whats wrong with tapwater?