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OA17

Member Since 18 Apr 2009
Offline Last Active Today, 04:56 AM
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#124132 Does it matter where a PhD is done?

Posted gebirgsziege on 22 November 2011 - 11:13 PM

although the ranking of your university seems to get more important, the decisive factor usually is the quality of your supervisor - and there are excellent supervisors who are young group leaders and bad supervisors who are well established senior scientist - and vice versa. Most important is that you think you can spend 4/5 years with your supervisor and the people in the lab - because I have seen many people choosing their supervisor by reputation who did hate their PhD and who were really unhappy in the groups.
So in my eyes its supervisor/group first, then topic, and then the institution. If you like what you are doing and where you are doing it you will be more productive which will in the end define your chances.


#145883 Questions / Help with Fusion PCR

Posted ipman_81 on 26 November 2012 - 03:26 PM

Finally got it to work like everyone else's!! Ok so it seems as though the mid-piece had some issues. I confirmed the primer sequence for amplifying the midpiece and did an experiment to test between what I had been using this whole time and a newly amplified midpiece fragment. The new ones came out almost flawless! =)

So I think the lesson is that even if people in the lab have done it a thousand times, make sure you understand the method thoroughly and double check everything that is handed down to you.


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