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moerae

Member Since 16 Apr 2009
Offline Last Active May 13 2013 06:53 PM

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Posts I've Made

In Topic: QC saga continues.... from bad to worse

25 March 2013 - 12:57 PM

I have dye-swapped and also checked the manual. Have run out of ideas.

In Topic: Nimblegen CGH array QC issues

04 March 2013 - 01:34 PM

Ok. We basically strip away the plastic chambers that keep the arrays separate from each other on the slide while it's in one of the wash buffers and once that's off we swish it around in there for a few seconds. Then the slide is transferred to a slide washing container with another wash buffer and this is shaken vigorously for 2 mins. This process is repeated twice more with different wash buffers before it's spun dry and scanned.

In Topic: Nimblegen CGH array QC issues

03 March 2013 - 05:40 PM

How do I know if the problem is caused by ozone?

As for proper draining... I swish it quite a few times in the initial wash solution before popping them into the slide containers to wash. I've basically done everything like the person who taught me and she has no or very little issues. The only difference is the DNA samples. As I wasn't provided with enough DNA to re-amplify, I've had the samples rehybridised by the person who taught me (so the labelled DNA is the same as what I have used), and some samples that did not pass QC passed the second time around and the ones that passed QC previously failed the second time. I'm very very puzzled by this.

In Topic: Nimblegen CGH array QC issues

27 February 2013 - 12:11 PM

We don't have a stabliser for our arrays. We just wash, dry and scan the array. The funny thing is that the AC drop outs isn't across the whole array, just random samples here and there, which is puzzling.

In Topic: PolyA tail essential?

29 October 2012 - 02:12 PM

Thanks for the reply. I'm going to do in vitro transcription using T7/T3 RNA polymerase and the kit I have only adds the 5' cap. Am I right to assume that I'll need a specific kit to add a polyA tail to the end of the RNA transcript?

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