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Thapa

Member Since 10 Apr 2009
Offline Last Active Feb 27 2013 09:52 AM

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Topics I've Started

overexpression using pcDNA3.0 in stably knockdown cells

04 July 2012 - 12:30 PM

Hi,
I have generated a stable clone using Lentiviral shRNA-mediated knockdown. The knockdown clones have suppressed expression of my target protein A.

Now, I want to rescue or re-express the same protein to compare its biological functions.

Can I use pcDNA3.0 vectors to overexpress the same target (protein A) in my stable clones?

Since my cells express siRNAs for the same target, dont they suppress pcDNA based overexpression of same gene?

Many thanks,