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kentz19

Member Since 07 Apr 2009
Offline Last Active Mar 30 2013 12:10 PM

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My research interests
pharmacology CNS & PNS, phytochemistry, biochemistry, biochemical pharmacology

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2xzwei
15 Apr 2013 - 04:15
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Topics I've Started

Km Vmax

22 March 2013 - 09:23 AM

Hello all,

I have few questions on this matter, let me give one example that is 'the reported Km value of glucose for GLUT1 in human is 10mM'.

My questions are:

1) Why do we need to refer to Km value and used that enzyme concentration in enzyme/transporter related work?
2) Will this glucose Km value be the same for all people or it varied?
3) Will this value vary across species ie human vs rat?
4) Will we get the same Km value in in vitro and in vivo experiment for GLUT1?
5) Can we simply take reported Km value and use it for experiment or it will be best for determining for each new experiment?

Looking forward to hear from you guys.

Many thanks.

Permeability coefficient unit, cm/s

28 August 2012 - 06:17 PM

Hello all,

I'm working with in vitro BBB model and using 14C-sucrose as a permeability marker. However, I'm not quite getting the concept of permeability coefficient unit, cm/s. I got a permeability coefficient value 2 x 10^-5cm/s. What does this means since cm is a unit for distance and not concentration. Can anyone explain about this?

Thank you

Pen/Strep from Sigma unit confusion

22 July 2012 - 07:44 AM

Dear all, I would like to ask about unit for Pen/Strep preparation from Sigma (P0781), 100 mL. On the label, the concentration is 10000 unit/10 mg/mL Pen/Strep. The 10000 unit of penicillin referred to the amount of penicillin present in 100 mL or the concentration (10000 unit/mL). Anyone can help me on this?