Jump to content

  • Log in with Facebook Log in with Twitter Log In with Google      Sign In   
  • Create Account

Ahrenhase

Member Since 03 Apr 2009
Offline Last Active Jun 04 2013 08:20 AM
-----

#155838 Is there a such thing that would allow me to generate 2 individual proteins, but

Posted Ahrenhase on 30 May 2013 - 05:21 PM

their expression in the same cell causes them to come together?  i.e. a molecular tool for creating endogenous fusion proteins


#153491 problems with plasmid miniprep

Posted Ahrenhase on 09 April 2013 - 12:14 PM

Most likely.  The genomic DNA should be pulled down with the flocculant after the addition of the potassium acetate.  You could be shearing it into pieces at those concentrations of NaOH.


#144886 How to unleash the creative mind after being "conditioned" to the academ

Posted Ahrenhase on 08 November 2012 - 07:54 PM

I'm coming to realize that just because you can memorize a bunch of random facts for a test and score excellent grades doesn't make you a good researcher.  Also, just because you're technically capable of conducting an experiment proficiently from beginning to end isn't the only quality of a good researcher.   I know the science (i.e. what's going on inside my tube) and I'm great with my hands; however, when it comes to determining what to do next I'm absolutely useless (unless it basic and obvious).  Oddly, I used to be very creative, but a lifetime of rote memorization and droning over busy work has depleted me to the point that I just feel uncreative, stressed, and anxious all the time.  Has anyone successfully decompressed from this mindset?


Home - About - Terms of Service - Privacy - Contact Us

©1999-2012 Protocol Online, All rights reserved.