and what is the different of the result of the native protein or the denatured protein from WB ?
or is there any meaning to interpret our results, if we use the native protein or the denatured protein?
denatured protein will give you subunit size.
some antibodies will only recognize denatured protein, some will only recognize native protein, and some will recognize both.
That mean, it depends on antibody that is available for our particular protein, right ?
Then, we can design to prepare the lysis buffer for native or denature sample.