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There are a few free pathway editing or drawing tools available.
http://kgml-ed.ipk-g...stallation.html
KGML-ED allows the dynamic exploration and editing of KEGG Pathway diagrams. It is a graphical network editor, that provides read- and write-support for the KGML (KEGG Markup Language) file format. Pathway files are loaded and transformed into a graph network which may be modified to fulfill user-specific needs (e.g. it is possible, to delete or add network elements, change labels and colors).
http://www.genmapp.o...wnload_v2.1.php
GenMAPP is a free computer application designed to visualize gene expression and other genomic data on maps representing biological pathways and groupings of genes. Integrated with GenMAPP are programs to perform a global analysis of gene expression or genomic data in the context of hundreds of pathway MAPPs and thousands of Gene Ontology Terms (MAPPFinder), import lists of genes/proteins to build new MAPPs (MAPPBuilder), and export archives of MAPPs and expression/genomic data to the web.
BioCart:
Biocarta does provide pathway templates for you to use with Freehand or illustrator.
http://www.biocarta....genes/index.asp
Download Pathway Tools
Below are links that will allow you to download the Biocarta templates which you can use in the creation of your own Pathways.
#127498 Pathway Analysis of Microarray data
Posted
on 22 January 2012 - 10:06 AM
#77759 FACS analysis of GFP expressing cells - Fix or not
Posted
on 01 July 2010 - 06:46 AM
I would suggest that you don't fix the cells and use them directly. I have GFP positive mice and I do FACS regularly for GFP +ve cells. I have also tried fixing the cells to stain for transcription factors. But once I fixed the cells, I find that GFP expression is lost and the cells look like WT GFP -ve cells. So now I don't look at GFP if I am doing fixation.
