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I am not sure if there is a time limit but I like to look at my cells right after staining. I am not very keen to leave cell suspension in stain for too long.
PI I use is dissolved in PBS, have to check percentage, on top of the head 1 %. And following staining cells need to be washed really well, PI gives lots of background.
Cells always have some autoflorescence so ideally stained healthy cells will be included as a control.
Oh, samples need to be RNase treated because PI binds to RNA, producing false positives.
#86629 Basic questions about PI staining in Flowcyto
Posted
on 11 September 2010 - 03:30 PM
