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Hi,
I'm doing phospho-(AKT, GSK3b) WB using Odyssey blocking buffer:PBS 1:1but only see very week signals, and some unspecific bands. I wonder what is the optimal blocking buffer to use. The instruction provided by Licor shows a WB figure comparing Odyssey blocking buffer:PBS 1:1, 5% BSA, etc, but I don't see much difference there. On the other hand I heard that BSA is better than the Odyssey blocking buffer. Does anyone have experience on this? Which BSA concentration to use? (I'm using nitrocellulose membrane)
Thanks in advance.
P
