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leelee

Member Since 09 Mar 2009
Offline Last Active Today, 07:42 AM
*****

Topics I've Started

Science, it works.... bitches.

02 April 2013 - 09:05 PM

Richard Dawkins on why we should trust the scientific method. I may not always agree with the guy, but love this :)


removing MHC class I from the cell surface

31 October 2012 - 07:48 PM

Hi all

Does anyone know if it is possible to strip MHC class I molecules from the surface of cells (enzymatically, chemically, whatever...)?

I'm having a hard time finding any information on this, as every search I perform comes up with info on stripping the peptides from the mhc I molecules, but not the molecules themselves.

Fingers crossed one of you can help!

Thanks

leelee Posted Image

cell synchronisation for fibroblasts (help choosing method/chemical)

11 September 2012 - 09:43 PM

Hi everyone,

I'm looking to synchronise my fibroblasts prior to infection with my virus, so that they are all equally susceptible to infection and replication of my virus.

I have no experience with synchronising cells and would love to hear your opinions on any methods you may have experience with. The stage of the cycle arrest is not particularly important to me.

Basically I just want something that is cost effective, efficient and will just work (story of my life...Posted Image )

So far I'm about to look into mimosine, lovastatin and nocodazole. I'd prefer to use a chemical inhibitor rather than serum starvation, as I've been told that can be quite unreliable (but feel free to set me straight).

Hope you can help

Thanks,

Leelee

can you recommend a gateway mammalian expression vector? Please? :)

12 June 2012 - 07:59 PM

Hi all,

I'm wanting to generate some stable cell lines expressing my gene of interest.
I plan to use BALB/c 3t3 fibroblasts.

I have the gene of interest as pDONR entry clones, and am about to go ahead and generate expression clones using pCDNA3-DEST. My PI has failed with this in the past which got me thinking...

Is there anything better available? What do you use, what would you suggest?

Any ideas and help appreciated.

Thanks!

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