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Hi all,
Sorry if this has been covered in previous posts- I can't seem to find the right search terms to locate them.
Anyway-
I'm staining my infected fibroblast cell line with an antibody against a viral protein. As this is obviously intracellular, I'm permeabilising my cells with 0.05% Tween 20 (in PBS with 2% NCS).
I find that after resuspending the cells in the perm buffer, they become very sticky and tend to clump together, making them difficult to resuspend as a single cell suspension.
Does anyone know if there is anything I can do to minimise or eliminate this problem- or is it just what happens once cells have been permeabilised?
Thanks!
