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Science, it works.... bitches.
02 April 2013 - 09:05 PM
Richard Dawkins on why we should trust the scientific method. I may not always agree with the guy, but love this
removing MHC class I from the cell surface
31 October 2012 - 07:48 PM
Hi all
Does anyone know if it is possible to strip MHC class I molecules from the surface of cells (enzymatically, chemically, whatever...)?
I'm having a hard time finding any information on this, as every search I perform comes up with info on stripping the peptides from the mhc I molecules, but not the molecules themselves.
Fingers crossed one of you can help!
Thanks
leelee
Does anyone know if it is possible to strip MHC class I molecules from the surface of cells (enzymatically, chemically, whatever...)?
I'm having a hard time finding any information on this, as every search I perform comes up with info on stripping the peptides from the mhc I molecules, but not the molecules themselves.
Fingers crossed one of you can help!
Thanks
leelee
cell synchronisation for fibroblasts (help choosing method/chemical)
11 September 2012 - 09:43 PM
Hi everyone,
I'm looking to synchronise my fibroblasts prior to infection with my virus, so that they are all equally susceptible to infection and replication of my virus.
I have no experience with synchronising cells and would love to hear your opinions on any methods you may have experience with. The stage of the cycle arrest is not particularly important to me.
Basically I just want something that is cost effective, efficient and will just work (story of my life...
)
So far I'm about to look into mimosine, lovastatin and nocodazole. I'd prefer to use a chemical inhibitor rather than serum starvation, as I've been told that can be quite unreliable (but feel free to set me straight).
Hope you can help
Thanks,
Leelee
I'm looking to synchronise my fibroblasts prior to infection with my virus, so that they are all equally susceptible to infection and replication of my virus.
I have no experience with synchronising cells and would love to hear your opinions on any methods you may have experience with. The stage of the cycle arrest is not particularly important to me.
Basically I just want something that is cost effective, efficient and will just work (story of my life...
)So far I'm about to look into mimosine, lovastatin and nocodazole. I'd prefer to use a chemical inhibitor rather than serum starvation, as I've been told that can be quite unreliable (but feel free to set me straight).
Hope you can help
Thanks,
Leelee
