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My student had cloned and expressed a capside protein from a bovine retrovirus (His-tagged) in the baculovirus system. She has got the expression correctly (checked by WB) but the cells look "punky". When we stained the cells with a FITC conjugated anti-His Mab (MACS Molecular), the cytoplasm and the "spikes" get fluorescent (see attached picture). We then stained with Alexa Fluor 488 phalloidin, and it looks the same, but red, with long convergent structures organized from the center of the cells, suggesting some kind of association of the viral protein with the cytoskeleton. Had anyone seen something like this before? Any comment or suggestions?
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Mab anti HIs.pdf 34.86K
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