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The formula you have given looks like it should be t=r/Sqrt((1-r^2)/(N—2)) the t statistic for testing if the correlation coefficient is significant, it can then be converted to a p value using the T.DIST function in Excel.
#154404 Method of calculating p value for Pearson's correlation coefficient
Posted
on 29 April 2013 - 03:37 PM
#145585 Calculating enzyme activity
Posted
on 20 November 2012 - 11:19 AM
You are on the right track; just go carefully through your units.
The first step is correct to get 3.7x10^-5 M
But this is over 60min so you have 6.17x10^-7 M/min
Then convert to uM/min
To get to enzyme units you need to remember uM is an abbreviation for umol/L so you can multiply out the assay volume to leave umol/min
Your standard curves of enzyme vs OD are important for demonstrating that you have a linear response wrt enzyme concentration.
The first step is correct to get 3.7x10^-5 M
But this is over 60min so you have 6.17x10^-7 M/min
Then convert to uM/min
To get to enzyme units you need to remember uM is an abbreviation for umol/L so you can multiply out the assay volume to leave umol/min
Your standard curves of enzyme vs OD are important for demonstrating that you have a linear response wrt enzyme concentration.
#140124 Drug recrystallize when i added to DMEM
Posted
on 28 August 2012 - 12:36 PM
sometimes disolving in FBS or BSA will help
#98337 [help] about plate reader wavelength
Posted
on 23 January 2011 - 02:06 PM
If it is the ‘lamp energy’ then you may need a new light source not a new filter.
But in addition to the recommendations above; you ought to be able to continue with the 405nm so long as you have an appropriate standard curve albeit with reduced readings and sensitivity.
good luck
But in addition to the recommendations above; you ought to be able to continue with the 405nm so long as you have an appropriate standard curve albeit with reduced readings and sensitivity.
good luck
