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Hi all! I have a question for you. I am looking at relative uptake of a compound between two cell types (microglia and astrocytes). Ideally, an equal number of cells would be treated at equal confluency, however astrocytes are quite a bit bigger than microglia. Therefore, if each plate is seeded with one million cells of either microglia or astrocytes, the astrocyte plate is going to appear much more confluent than the microglial plate. Likewise, an 80% confluent plate will have many more microglia than astrocytes.
Which situation would you consider preferable? Equal confluency (ie, both treated at 80% confluency) or equal number of cells (ie, 1 million cells treated regardless of confluency)?
Thanks!
