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knuf

Member Since 05 Feb 2009
Offline Last Active Dec 04 2012 10:14 AM
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Posts I've Made

In Topic: Dot blotting problems

13 July 2012 - 06:16 AM

whenever i do dot blots i use nitrocellulose membrane...then you don't have to worry about wet/dry issues

In Topic: IHC: confirming specificity phosphorylated protein antibody

13 July 2012 - 06:14 AM

Just as an addition to what Bob said (which are all good suggestions) with the peptide competition assay, you should see decreased reactivity when pre-incubated with the phospho-peptide but not with the unphosphorylated peptide

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