Jump to content

  • Log in with Facebook Log in with Twitter Log In with Google      Sign In   
  • Create Account
  2. → Viewing Profile: Topics: chimera

chimera

Member Since 03 Feb 2009
Offline Last Active Dec 14 2012 07:30 AM
-----

Topics I've Started

Immunoprecipitated protein visible on coomassie but not detected by Western

14 December 2012 - 03:42 AM

Hi everybody,
                     I am immunoprecipitating my protein with its antibody. I run the eluted proteins onto the gel and could see the immunoprecipited bans on coomassie. But I could not detect the protein by western analysis. What could be the possible explanation for this bizzare phenomena? Please help me. Lots of thanks in advance.

How to elute antigen during immunoprecipitation experiment with out primary anti

13 December 2012 - 11:47 PM

My protein (50 kDa), which I am trying to immunoprecipitate matches with the heavy chain of primary antibody. So I want to elute only my antigen without disturbing the primary antibody, so that I can detect it on the western. How can I do that? Please help me. Lots of thanks in advance.

Mass-spec compatible silver stain

04 December 2012 - 08:45 AM

Want to do mass spec analysis of a group proteins, which are of extremely low quantity. Can anybody suggest me any good mass-spec compatible silver staining kit for SDS gels? Lots of thanks in advance.

Shift in the position of band onto the western blot

16 October 2012 - 10:13 PM

A predicted band of 45 kDa appeared in the position of 95 kDa on the western blot . Shift in the position is a very general phenomena for  the group of proteins it belongs to. Could you guys help me to explain it? What kind of post-translational modification can be guessed? Please help me.

  1. BioForum
  2. → Viewing Profile: Topics: chimera
  3. Privacy Policy
Home - About - Terms of Service - Privacy - Contact Us

©1999-2012 Protocol Online, All rights reserved.