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Trof, on 20 July 2012 - 06:17 AM, said:
I have a similar question. I used Sigma-Aldrich Imprint CHIP kit, but those DNA isolation columns in kit were awfull, hard to open, uneven size (some of them didn't fit in the centrifuge), crappy plastics and what's more important the elution volumes were different and about only 2/3 of the volume I put in. Couldn't believe that.
If I need to repeat it, I would rather use other columns. We have Qiagen MinElute and Qiaquick columns (various kits, Gel Extraction, PCR purification maybe even reaction cleanup) and DNeasy Blood and Tissue colums. Which do you think would be most suitable for CHIP DNA purification? I'm mostly worried about buffer compatibility (CHIP lysate with column binding conditions) in that case.
If I need to repeat it, I would rather use other columns. We have Qiagen MinElute and Qiaquick columns (various kits, Gel Extraction, PCR purification maybe even reaction cleanup) and DNeasy Blood and Tissue colums. Which do you think would be most suitable for CHIP DNA purification? I'm mostly worried about buffer compatibility (CHIP lysate with column binding conditions) in that case.
I would think, given the small yield in a ChIP assay, that the MinElute kit would be most appropriate.
