I have another question regarding this nice project. Do you have an idea what the maximum distance between my specific protein and my epitope tag could be. I have the problem that I can not ligate the gene directly with the epitope sequence. So there are minimum 12 bases in between. I often don't see any information in other papers. One showed 6 bases.
Example for C-terminal tagging:
Would be great if somebody has an idea.
MontysMember Since 15 Apr 2001
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