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Curtis

Member Since 30 Jan 2009
Offline Last Active Today, 11:00 AM
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Posts I've Made

In Topic: Promoter distance from ATG

Yesterday, 07:50 PM

that is far, think of an alternative.

In Topic: faint band after plasmid extraction

12 June 2013 - 07:11 AM

It's kanamycin. I also think the induction solution must be IPTG. It's written 1000x on the tube.

In Topic: faint band after plasmid extraction

11 June 2013 - 10:08 PM

The company just replied that the bacteria are JM108,  not TOP10. They made a mistake in the data sheet. Really useless staff. They also forgot to sent me the protocol of induction. Can you tell me the protocol?

In Topic: faint band after plasmid extraction

11 June 2013 - 08:56 AM

The company sent me the plasmid in a separate tube as well. An induction solution was also included. Not sure how to use that. I really hope I can transform it. Last week we transformed 15k to electro dh10b without any problem...thanks guys.

In Topic: choosing the correct subculturing ratio - ovarian cancer cell lines

10 June 2013 - 01:39 AM

If it's an immortal cell, then yes you can. We usually split 1:10 if we want to just subculture for later use. But we split with a bigger ratio if we have a lot of transfection to do in the week and need more cells. Most cells need 24 h to rest after seeding and before transfection.

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