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In Topic: Promoter distance from ATG
Yesterday, 07:50 PM
that is far, think of an alternative.
In Topic: faint band after plasmid extraction
12 June 2013 - 07:11 AM
It's kanamycin. I also think the induction solution must be IPTG. It's written 1000x on the tube.
In Topic: faint band after plasmid extraction
11 June 2013 - 10:08 PM
The company just replied that the bacteria are JM108, not TOP10. They made a mistake in the data sheet. Really useless staff. They also forgot to sent me the protocol of induction. Can you tell me the protocol?
In Topic: faint band after plasmid extraction
11 June 2013 - 08:56 AM
The company sent me the plasmid in a separate tube as well. An induction solution was also included. Not sure how to use that. I really hope I can transform it. Last week we transformed 15k to electro dh10b without any problem...thanks guys.
In Topic: choosing the correct subculturing ratio - ovarian cancer cell lines
10 June 2013 - 01:39 AM
If it's an immortal cell, then yes you can. We usually split 1:10 if we want to just subculture for later use. But we split with a bigger ratio if we have a lot of transfection to do in the week and need more cells. Most cells need 24 h to rest after seeding and before transfection.
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