I doubt that this is bacterial contamination- the media would be cloudy and the dots would be much, much smaller than the cells and probably moving.
The overall appearance of the fibroblasts are not very happy looking- there are lots of vacuoles and I suspect your cells are either apoptosing or necrosing.
If it is an infection, I would say it looks possibly like a fungal infection.
I would throw these cells and start again with fresh media and give your incubator and hoods a good clean out (if you're not already doing that).
Sorry, but I hope that helps
LostintheLabMember Since 28 Jan 2009
Offline Last Active Feb 15 2013 07:15 AM
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