Find content
Male
if you mix 1ml of 20uM forward primer with 1ml of 20uM reverse primer then you will have 2ml of 20uM primer mix but 10uM each primer.
#100607 final concentration
Posted
on 14 February 2011 - 01:40 PM
#100253 Osmolarity
Posted
on 10 February 2011 - 05:23 PM
Think about it like this... 1 mole of NaCl goes into solution to form one mole of Na+ and one mole of Cl- = 2 Osmole... HEPES and glucose do not do this so the osmolarity is 1 for each mole dissolved.
Assuming your solution above is in millimoles/litre (you should always write solutions with concentration units)
160 mM NaCl = 320 mOsmole
2.5 mM KCl = 5 mOsmole... etc I am sure you get the picture.
BTW Inmost sun is correct...
Assuming your solution above is in millimoles/litre (you should always write solutions with concentration units)
160 mM NaCl = 320 mOsmole
2.5 mM KCl = 5 mOsmole... etc I am sure you get the picture.
BTW Inmost sun is correct...
#21820 how do I generate a growth curve?
Posted
on 14 April 2009 - 10:11 AM
Like Aimkinks said: you need to count the cells at certain time-intervals and plot it out on a graph.
The following links may be helpfull:
link1
link2
link3
link4
how are you going to count te cells ? If you use alamar blue by example you can simply use the manual that you can order or get with the product , it explains how you need to do so.
The following links may be helpfull:
link1
link2
link3
link4
how are you going to count te cells ? If you use alamar blue by example you can simply use the manual that you can order or get with the product , it explains how you need to do so.
#100130 Does 90C moist heat incubator cleaning cycle really kill fungus?
Posted
on 09 February 2011 - 06:39 PM
I don't think those cycles will kill fungal spores at all, you would need autoclave sort of temperatures and pressures to do that.
#99773 Plasmid Question
Posted
on 06 February 2011 - 02:22 PM
Descibe how the size and topolgical constriction of plasmid facilate its purification?
#87179 E.Coli growth at 4 degrees?
Posted
on 17 September 2010 - 05:31 AM
This doesn't have anything to do with your refrigerator -- you're seeing satellite colonies arising on the plate.
When you plate a transformation mixture, it contains many cells that haven't been transformed, and (hopefully) some that have. Initially, these cells without a plasmid don't grow because of the ampicillin in the media. However, as the transformed cells grow, they secrete beta-lactamase (the enzyme that destroys ampicillin and confers resistance on the transformants). As this enzyme accumulates in the media and diffuses outward, the concentration of ampicillin drops, because the enzyme is destroying the ampicillin. Now the untransformed cells can grow, as the concentration of ampicillin remaining is too low to stop their growth.
So, yes -- E. coli does grow at 4 degrees, and you shouldn't store your transformation plates directly, rather you should pick your transformants to a fresh plate and store that.
When you plate a transformation mixture, it contains many cells that haven't been transformed, and (hopefully) some that have. Initially, these cells without a plasmid don't grow because of the ampicillin in the media. However, as the transformed cells grow, they secrete beta-lactamase (the enzyme that destroys ampicillin and confers resistance on the transformants). As this enzyme accumulates in the media and diffuses outward, the concentration of ampicillin drops, because the enzyme is destroying the ampicillin. Now the untransformed cells can grow, as the concentration of ampicillin remaining is too low to stop their growth.
So, yes -- E. coli does grow at 4 degrees, and you shouldn't store your transformation plates directly, rather you should pick your transformants to a fresh plate and store that.
#85425 Breast Feeding and Ethiduim Bromide
Posted
on 01 September 2010 - 01:04 AM
Well, I guess as breast milk does have shed cells and immune cells in it, there is some very small (infinetismal I would have thought) risk of passing a mutation on to a breast-fed child. I would have thought that there is unlikely to be any "free" EtBr on the body unless the person mamanges to get a large amount on themselves.
#85440 Breast Feeding and Ethiduim Bromide
Posted
on 01 September 2010 - 03:48 AM
#85039 How to clone DNA (already cloned in a vector) in to another vector
Posted
on 28 August 2010 - 07:01 AM
There is a map of pCMV6-XL4 here, and one of PCDNA1.1/AMP here.
The basic steps are to release your insert from its current vector using appropriate restriction enzyme(s), purify the released fragment from the pCMV6-XL4 backbone, and ligate the insert into appropriately digested PCDNA1.1/AMP, and recover the clones via chemical transformation or electroporation of a suitable bacterial host, selecting for successful transformants using the ampicillin resistance marker carried by PCDNA1.1/AMP.
The basic steps are to release your insert from its current vector using appropriate restriction enzyme(s), purify the released fragment from the pCMV6-XL4 backbone, and ligate the insert into appropriately digested PCDNA1.1/AMP, and recover the clones via chemical transformation or electroporation of a suitable bacterial host, selecting for successful transformants using the ampicillin resistance marker carried by PCDNA1.1/AMP.
#84848 +/- rating system
Posted
on 26 August 2010 - 08:32 AM
gebirgsziege, on 25 August 2010 - 10:40 PM, said:
Hi Biofourm,
is there a chance that the +/- rating for posts will be activated?
It would be very useful to strengthen very good answers. Often topics are sufficiently answered already with one single answer that needs nothing to add, but when a second user rates the answer as "+" this would add more impact and vice versa (although of course when rating an answer as "-" everybody should argument this and give a better one).
Thanks!
edit: bad spelling day
is there a chance that the +/- rating for posts will be activated?
It would be very useful to strengthen very good answers. Often topics are sufficiently answered already with one single answer that needs nothing to add, but when a second user rates the answer as "+" this would add more impact and vice versa (although of course when rating an answer as "-" everybody should argument this and give a better one).
Thanks!
edit: bad spelling day
I see where you are going to, but on the other hand: if someone read an answer and sees this as correct: he or she can easly type: ² or agree or...
If the user doenst do this, I doubt he or she would be willing to push some + button too..
Another problem would be the fact that if its like you say then no one will know who pushed the + button.. any fool can push it... or any idiot can push the - button.
Maybe an alternative would be that people can push a + button stating they agree and that you see their name (lets say, if you push the + button it simply shows a very short message: user XXX agrees with...).
#85221 Dilution with %
Posted
on 30 August 2010 - 11:34 AM
sansub, on 30 August 2010 - 10:14 AM, said:
I want to make 100 mls of 5% glutaraldehyde from 50% glutaraldehyde. Is it ok to take 10 mls of 50% glutaraldehyde and bring it to a final volume of 100 mls?
I want to confirm if this is correct.
Thanks!
I want to confirm if this is correct.
Thanks!
Yes, that's correct.
