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So, my second course Medical Neuroscience started over a month ago, but unfortunately I spent that month in hospital without good internet access. I pretty much regret that, since this course seems to be pretty thorough, with group assigments and other interactive stuff, and planned for and extensive period of 8 weeks. Now I can only watch the videos, without attempting Statement of Accomplishment because some of the weekly quizes are already overdue.
The course stuff said they plan to repeat the course in the future, so maybe then.
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Community Stats
- Group Global Moderators
- Active Posts 943
- Profile Views 13,564
- Member Title Brain on a stick
- Age 30 years old
- Birthday June 14, 1982
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Gender
Female
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Location
Czech Republic
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Hobbies
Gliding, computers, webdesign
About me
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Lab or Group
Dept. of Biology
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Institution
Palacky University Olomouc
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My research interests
Molecular hematology, real-time PCR
Posts I've Made
In Topic: Courses you are taking just now
Yesterday, 12:23 PM
In Topic: Point mutations in synthesis order of a 2.4 kb nucleotide fragment
Yesterday, 10:39 AM
You could synthesize it in two parts (with overlaping fragments) in E. coli, or use a modular system of artificially synthetized smaller fragments, that would be complementary (now I just forgot the exact name of this type of cloning stategy, even if you killed me..)
Something like this: A modular assembly cloning technique, for example.
But if the sequence is indeed toxic to E.coli, and you want to use it in E.coli, you will probably face the same problem (but as I understood, you want it for Lactococcus, so maybe this is not an issue).
Something like this: A modular assembly cloning technique, for example.
But if the sequence is indeed toxic to E.coli, and you want to use it in E.coli, you will probably face the same problem (but as I understood, you want it for Lactococcus, so maybe this is not an issue).
In Topic: Science, it works.... bitches.
09 April 2013 - 08:41 AM
It also entered my wardrobe this Christmas. I wore it at a lab seminar presentation. It was cool.
In Topic: Ambigious about the acceptance
09 April 2013 - 07:49 AM
In Topic: the storage time for primers
08 April 2013 - 11:02 AM
I was using stock batches of primers many years old.
But also, one time the stock diluted only in water, kept in -20 all the time, thawed only few times.. got completely degraded.
I use only 10mM Tris pH8 to dilute primers since.
But also, one time the stock diluted only in water, kept in -20 all the time, thawed only few times.. got completely degraded.
I use only 10mM Tris pH8 to dilute primers since.
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