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but I guess the pure alcohol bottle from your lab closet is disappearing on Fridays faster than usual, eh?
#125925 Words, Words, Words
Posted
on 22 December 2011 - 12:26 PM
junk food
but I guess the pure alcohol bottle from your lab closet is disappearing on Fridays faster than usual, eh?
but I guess the pure alcohol bottle from your lab closet is disappearing on Fridays faster than usual, eh?
#125202 Need help for PCR for AT rich gene
Posted
on 10 December 2011 - 11:05 AM
Often it is suggested to use betaine to make a PCR work in AT-rich regions. Also the companies say this about their additives (Q-solution (= betaine), PCRx Enhancer Solution etc). No idea if it's true, but an attempt might be useful.
#122978 Self-plagiarism?
Posted
on 03 November 2011 - 08:47 AM
What do you mean with "the chapter" of your thesis? Anyway if it's one chapter of several it should be okay, because an undergrad study can be a good starting point of a phd thesis. But it shouldn't be too much (the extent of repetition is critical here) and it anyway makes not much sense to do the same work again??
#121883 I was plagiarized, what should I do?
Posted
on 19 October 2011 - 08:12 AM
also as decent researcher you need as many papers as possible and poster publications help too...therefore I'd insist on my rights here...
#121229 salts in PCR
Posted
on 10 October 2011 - 08:36 AM
Cations stabilize the duplex state of DNA, and the algorithms for salt correction formulas are developed using Na+concentrations, for whatever reasons. So I'd take into account monovalent and bivalent ions as primer3 does it. I guess it's not that important if it's K+ or Na+ (someone might correct me here).
NH4+ surely also will have an effect as it is a cation, but it also influences the enzyme's activity. I don't know any program that accounts for that, but perhaps no data are available, as such additives are anyway a trial and error issue.
Actually my way to deal with it is to calculate roughly an annealing temperature (using standard settings) and then later doing a gradient PCR, if the reaction works. The calculated Tms are also always an estimate that have to be verified and then optimised. And also with simple formulas such as
Tm (°C) = 81.5 + 0.41(%GC) - (675/N) or Tm = 4(G + C) + 2(A + T) °C PCRs worked...
NH4+ surely also will have an effect as it is a cation, but it also influences the enzyme's activity. I don't know any program that accounts for that, but perhaps no data are available, as such additives are anyway a trial and error issue.
Actually my way to deal with it is to calculate roughly an annealing temperature (using standard settings) and then later doing a gradient PCR, if the reaction works. The calculated Tms are also always an estimate that have to be verified and then optimised. And also with simple formulas such as
Tm (°C) = 81.5 + 0.41(%GC) - (675/N) or Tm = 4(G + C) + 2(A + T) °C PCRs worked...
#118904 an alternative way for money
Posted
on 04 September 2011 - 02:14 AM
Writing proposals, outlines for grants and projects is a tiresome duty if you want to stay in research and have no permanent position or need additional money for a project....
as an alternative way to get money crowd funding becomes more and more important, and next to websites where this is offered for all types of projects, also sites that are only for funding science projects are created.
Here is a short paper about this in Nature Neuroscience.
A few examples:
Kickstarter is a website for all kinds of projects and ideas that need money (arts projects, start up companies, etc)
Fundscience (non-profit, USA-based, only for science projects)
MyProjects (UK, only for cancer research projects)
Open Genius (non-profit, science only, still in development I think)
Because of the not that high amounts of money you can get, it's perhaps a good idea for pilot projects, feasibility studies or funding gaps. Have a look.
as an alternative way to get money crowd funding becomes more and more important, and next to websites where this is offered for all types of projects, also sites that are only for funding science projects are created.
Here is a short paper about this in Nature Neuroscience.
A few examples:
Kickstarter is a website for all kinds of projects and ideas that need money (arts projects, start up companies, etc)
Fundscience (non-profit, USA-based, only for science projects)
MyProjects (UK, only for cancer research projects)
Open Genius (non-profit, science only, still in development I think)
Because of the not that high amounts of money you can get, it's perhaps a good idea for pilot projects, feasibility studies or funding gaps. Have a look.
#118223 Friday, I'm in love
Posted
on 25 August 2011 - 11:48 AM
And here an adapted version of an old song from Alice Cooper: School's Out
Well we're not choosy
All the nerds and geeks
bein' all that nosey
'Cause they found no sheiks
Well they can't fund ya
Can't find work
If that don't suit ya
Try to smirk
Lab's out for summer
Lab's out forever
Lab's been blown to pieces
No more pipets
No more papers
No more student’s dirty coats
Well we got no class
And we got no results
And we got no publication
We can't even think of a word that rhymes
Lab's out for summer
Lab's out forever
Lab's been blown to pieces
No more enzymes
No more digests
No more technician's dirty coats
Out for summer
Out till fall
We might not go back at all
Lab's out forever
Lab's out for summer
Lab's out with fever
Lab's out completely
Well we're not choosy
All the nerds and geeks
bein' all that nosey
'Cause they found no sheiks
Well they can't fund ya
Can't find work
If that don't suit ya
Try to smirk
Lab's out for summer
Lab's out forever
Lab's been blown to pieces
No more pipets
No more papers
No more student’s dirty coats
Well we got no class
And we got no results
And we got no publication
We can't even think of a word that rhymes
Lab's out for summer
Lab's out forever
Lab's been blown to pieces
No more enzymes
No more digests
No more technician's dirty coats
Out for summer
Out till fall
We might not go back at all
Lab's out forever
Lab's out for summer
Lab's out with fever
Lab's out completely
#116984 experiments in progress
Posted
on 09 August 2011 - 09:01 AM
#114381 How to open *.bin?
Posted
on 06 July 2011 - 08:46 AM
pito, on 06 July 2011 - 07:12 AM, said:
you need isobuster for it.
http://www.smart-pro...terdownload.php
and maybe this will help too:
http://www.ehow.com/...n-bin-file.html
http://www.smart-pro...terdownload.php
and maybe this will help too:
http://www.ehow.com/...n-bin-file.html
#110281 Musca domestica gDNA isolation
Posted
on 19 May 2011 - 10:56 AM
RNA? There can be surprisingly much and large in insects. Add RNAse and see if the band is still there.
#110081 Micropipetting technique
Posted
on 17 May 2011 - 09:02 AM
using a balance to control pipetting was suggested several times before
here is a powerpoint presentation from Rainin with several hints and some good advice:
raininpipettetechniques.ppt 1.83MB
164 downloads
here is a powerpoint presentation from Rainin with several hints and some good advice:
raininpipettetechniques.ppt 1.83MB
164 downloads
#107851 Words, Words, Words
Posted
on 24 April 2011 - 12:45 PM
nice theory
why doesn't it matter? and next week I'll diet I guess...
why doesn't it matter? and next week I'll diet I guess...
#106241 Nanodrop DNA
Posted
on 08 April 2011 - 12:01 PM
#103867 Placing dNTP in 64 C waterbath
Posted
on 16 March 2011 - 10:31 AM
I never did it, but don't think it will ruin it. The PCR reaction has higher temperatures...
Of course it depends on the time there, storing it at that temp will be harmful of course.
Of course it depends on the time there, storing it at that temp will be harmful of course.
#100823 Casting 3% agarose
Posted
on 16 February 2011 - 07:07 AM
If it's not the too hot temperature itself causing the cracks, but the temperature differences between hot gel and cold tray (leading to tensions within the tray material), you might preheat the tray slowly e.g. in a water bath.
