hobglobin

For all the gambling addicts, protein scientists and people who have sufficient time and like it to play, but also like it to help scientists dealing with protein folding issues:


FOLDIT


FoldIt is a game in which humans try to solve one of the hardest computational problems in biology: protein folding. You don't need to know anything about biology to play the game, although a little background will help. Download the software, install it and play.
The application displays a graphical representation of the protein's structure which the user is able to manipulate with a set of tools.
As the structure is modified, a "score" is calculated based on how well-folded the protein is, and a list of high scores for each puzzle is maintained. Foldit users may create and join groups, and share puzzle solutions with each other; a separate list of group high scores is maintained.

Example image of the game interface:

"Let your food be your medicine, and your medicine be your food" –Hippocrates Cure and healing can only happen when medicines are taken along with proper diet. If food and proper care is  neglected then it effects the body adversely. A proper ayurvedic diet along with herbal supplemennts help the body  to remain toned and fit. Diet however should vary along with the seasons. The reason for this is that every season  brings different types of illness and diseases. The immunity and resistance level of human body should vary with it.  Achieving a perfectly immune body is no child's play and asks for a planned diet and lifestyle.

The secret to perfect health is maintaining the equilibrium between human body and Mother Nature. Nature's power and   energy is always under constant reaction with human body because every living and non living thing is not a  stand-alone being but an extension of  the Creation. The five elements of Nature which are the main source of the  sustainance of life forms an integral part of the nutrition level of the food. Thus, food taken according to dietic  rules leads to cure and proper health.

In Ayurveda, the diet forms main part of the treatment for any ailment. The cure for any disease cannot be achieved  only by intake of medicines but depends on the healing power of food. Ayurvedic practitioners greatly rely upon the  mutual reaction of food, medicine, excercise and proper lifestyle which together bring upon physical, mental and  spiritual healing.

The real toxicity and hazard of EtBr seems still questionable, though all the classifications (MSDS et al) classify it this way. And I also agree that one should be careful and wear gloves, and don't use powders of it. Anyway there's also some hysteria about this substance, read this article about it. An useful and reasonable text.
Anyway many of the dyes are more or less dangerous as they all interact in some way with your DNA and can penetrate through your skin and gloves in different speeds, or are later metabolised to toxic products. Here is an unmasking blog about SYBRsafe...
I guess silver is a quite safe alternative (though the developer and fixation substances might be not). Crystal violet and some other dyes might be also an alternative with less toxicity, read this pdf.
Read also this thread from here. Swanny convinced me somehow...

...and now to continue my own monologue...ok, so you need to add fluoresceine if using the Bio-Rad cyclers bec the SybrGreen signal is not strong enough for the machine to calculate background levels for normalisation iow as a reference dye ....thanks for the info...and one last question, why such a concern for freezing your own master mix when most of the commercial mixes are stored frozen before use anyways?

Hudson: I thought your weapons of choice were the fang and the claw.

Gargoyles
well, you're hard of hearing so I can't blame you...

AUSA Caroline Julian: Eat! Last time your stomach was growling louder than your testimony.

Bones
swooning might be the only way to survive that...oh I forgot vomiting of course