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could be proteases. some cleave at the terminus, some have very specific cleavage sites.
there are a number of ptms possible in e coli (eg proteolysis, phosphorylation,...). some are engineered into some strains (eg glycosylation).
phosphorylation could make a protein migrate faster in sds as well as normal page (and especially in urea-page).
since the mass scores are different, you can sequence the proteins to determine if there are any changes in the primary structure.
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In Topic: Trouble with purified recombinant protein bands
Today, 10:25 AM
In Topic: Glycocalyx is unmanifest
Today, 08:32 AM
i would think, without reading the paper for the context, that the authors were saying that they didn't see it. they probably should have said that it was "not manifest" rather than unmanifest (which, to me, would mean that it was lost or no longer present due to the conditions...).
In Topic: Trouble with purified recombinant protein bands
Today, 06:26 AM
are there any differences in the mass fingerprints? you may have a slightly truncated version of the protein or different his tag lengths or post-translational modification.
differences of mobility in native page can be due to charge as well as size differences (or a combination).
differences of mobility in native page can be due to charge as well as size differences (or a combination).
In Topic: Glycocalyx is unmanifest
Today, 05:57 AM
according to wikipedia english:
The unmanifested is the Absolute, the pure and formless ground of being from which creation and manifestation arise. As such, the unmanifested is free from change, the unmoved mover. It also, necessarily, cannot be explained or comprehended in terms of any manifest reality.
The unmanifested is the Absolute, the pure and formless ground of being from which creation and manifestation arise. As such, the unmanifested is free from change, the unmoved mover. It also, necessarily, cannot be explained or comprehended in terms of any manifest reality.
In Topic: CO2 to sedate animals
Yesterday, 04:12 AM
it will kill the animal. one of my neighbors used to use co2 to sacrifice his animals so that there would be no drug induced alterations to the brain.
the animal pants then falls asleep. if you don't take it from the co2 chamber, it will die.
the animal pants then falls asleep. if you don't take it from the co2 chamber, it will die.
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