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gfischer

Member Since 26 Jan 2009
Offline Last Active Yesterday, 12:34 PM
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#145916 Neurosurgery

Posted gfischer on 27 November 2012 - 09:00 AM

Screws made from titanium would be compatible with MRI (titanium is a nonferrous metal, so doesn't affect MRI).  You would obviously, however, have to be extremely careful not to damage the brain in the process.


#140411 What gel percent and Voltage is needed to separate 2680bp and 2600bp DNA nicely

Posted gfischer on 31 August 2012 - 08:33 AM

I think you're going to find it very difficult to get complete separation of fragments that close in size.

Assuming this is a restriction enzyme digest, an easier solution would be to add another enzyme to cut the fragment you don't need into two smaller fragments, so you can more easily separate them from your band of interest.


#110057 What does metaplasticity mean?

Posted gfischer on 17 May 2011 - 06:27 AM

I believe it refers to changes in the ability of neurons to undergo the changes in their firing properties typical of synaptic plasticity.  It's basically "plasticity of synaptic plasticity"


#97429 Transgenic mouse model to measure Ca2+ in confocal

Posted gfischer on 13 January 2011 - 10:24 AM

I'm not completely sure why you're looking at transgenic models for intracellular calcium measurements.  Fura-2 is a standard ionophore for measuring calcium levels in cells in response to chemical stimuli.  You might also look at Cameleon, a CamKII-GFP hybrid protein that can be transfected or transduced into cells to visualize calcium concentration.


#85221 Dilution with %

Posted gfischer on 30 August 2010 - 11:34 AM

View Postsansub, on 30 August 2010 - 10:14 AM, said:

I want to make 100 mls of 5% glutaraldehyde from 50% glutaraldehyde. Is it ok to take 10 mls of 50% glutaraldehyde and bring it to a final volume of 100 mls?
I want to confirm if this is correct.

Thanks!

Yes, that's correct.


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