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little mouse

Member Since 26 Jan 2009
Offline Last Active Feb 17 2012 06:57 AM
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Posts I've Made

In Topic: Enzyme: Taq stability

23 November 2011 - 08:17 AM

I forgot Taq on the bench overnight.
It still worked.

It is better to keep it at -20°C, but if once you forgot it, you don't have to trash it. try with one or two samples to see if it works.
If I were your boss, I would not panic, but still I would shout on you not to forgot the enzyme on the bench. ;)

In Topic: sequencing trouble

17 November 2011 - 08:00 AM

are you sure about the orientation of your primers?

In Topic: developing film manually

20 September 2011 - 10:47 PM

"These antibodies were raised against sheep."
It means that it recognizes a sheep protein, isn't it? I would say it is an anti-sheep, maybe
you should use an antibody raised in sheep against your phosphorylated protein , with your anti-sheep secondary antibody.

In Topic: developing film manually

20 September 2011 - 03:26 AM

I heat my samples ... I have to check now if it is 2 minutes only, usually I do 5 minutes. I have to go back to my lab books for the phosphorylations.

In Topic: developing film manually

19 September 2011 - 11:03 PM

Ok, the acetate sheet is the saran film I was talking about,

and yes, don't dry the membrane, but remove the excess of ECL as said almost a doctor, "by just lifting the membrane with a pair of tweezers and allowing the ECL to flow down into a piece of tissue paper."
He speaks english better than me.

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