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neuron

Member Since 26 Jan 2009
Offline Last Active Apr 11 2013 08:17 PM

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Topics I've Started

most common amino acid

27 March 2013 - 02:40 AM

Hey anybody has some idea about which is the most common amino acid in mammalian proteins? I know it is a very broad question. But still are there any studies like this?

what does this mean-

"To the solution X was added a solution of 5 eq. of Y in 10 μl THF. The reaction was kept at room temperature for 6 hours. Next, the product was isolated using preparative HPLC to give 15 O.D._A260of compound".

what is 15 O.D.A260? As far as I know about HPLC, we collect fractions based on the retention time, how do collect fractions based on OD?

column chromatography

03 March 2013 - 11:03 PM

What calculations do we use for packing any column.i.e. how much volume of beads should be taken for how much compound? For different types of adsorbents, the calculation vary. Like when we run silica gel column we take 1gm slurry for 10mg compound (???)...what about c18, g25 etc.?..I have tried to find out on google for some basic calculation..but did not succeed.. Posted Image

..so experts please help me, I am confused about column chromatography Posted Image

Instruction manual for TL-100 ultracentrifuge

21 February 2013 - 02:19 AM

Anyone has the instruction manual for TL-100 ultracentrifuge? Its very old model, I am not able to find it on google Posted Image

Confirmation of ligation

18 February 2013 - 11:16 PM

After ligation generally we do restriction digestion to confirm if we have got the clones and if the insert is ligated to the vector. But I found on google that many people also do PCR for confirmation. My question is, if the primers spanning to only insert can also be used for this? Whatever colonies we see on plate will be having some resistance, so either it could be ligated plasmid or empty plasmid. But could it be "empty plasmid and insert both in one colony"?? Posted Image

. Then this will also give amplification?

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