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...But my question is when we do cloning in an expression vector. we just put our DNA sequence ahead of promoter sequence, transcription and translation are taken care in bacteria. Therefore when you transform your vector into bacteria, it forms mRNA first and then DNA..so to make mRNA also promoter is required?????
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In Topic: Are two promoters needed for co-expressing 2 proteins from the same plasmid in S
11 April 2013 - 08:16 PM
I completely agree with you donny. I mixed translation and transcription ...But my question is when we do cloning in an expression vector. we just put our DNA sequence ahead of promoter sequence, transcription and translation are taken care in bacteria. Therefore when you transform your vector into bacteria, it forms mRNA first and then DNA..so to make mRNA also promoter is required?????
...But my question is when we do cloning in an expression vector. we just put our DNA sequence ahead of promoter sequence, transcription and translation are taken care in bacteria. Therefore when you transform your vector into bacteria, it forms mRNA first and then DNA..so to make mRNA also promoter is required?????
In Topic: Are two promoters needed for co-expressing 2 proteins from the same plasmid in S
10 April 2013 - 01:39 AM
Yes It is essential, In case of E.coli also, I think it is essential. For instance pETduet vector which is used to coexpress two genes in e.coli also has two separate promoters . Theoretically also if we see, translation would stop after reaching to stop codon, then to start the translation for second gene, where will polymerase bind?..there has to be some promoter sequence for that....I hope I am making sense (I may be wrong though
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