It has been a case of "I did warn you."
I have attempted to improve the efficiency of my multiway ligation by using a different ligase, a higher fidelity ligase. The only other ligase sold commercially is E.coli ligase. It is stated that E. coli does have higher fidelity compared to T4 ligase, however NEB did warn that...
perneseblueMember Since 26 Jan 2009
Offline Last Active Jul 09 2012 04:45 PM
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