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ForEpi

Member Since 26 Jan 2009
Offline Last Active May 16 2013 12:33 AM
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Topics I've Started

cytosine methylation stability

16 October 2012 - 06:29 AM

Anyone have any idea how stable cytosine methylation in vivo is when subjected to environmental insults? What product it converts to over time? I assume it deaminates into thymidine? Any idea how long that it takes under which conditions?

Thanks,

ForEpi

Spin columns with bisulfite conversion

06 June 2012 - 10:37 PM

Hi,

The Qiagen Epitect kit comes with 48 spin columns and enough bisulfite conversion reagents for 60 conversions (6x10 samples). Has anyone tried using the protocol on other spin columns?

Cheers

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