I did not do any experiment after above attempt.
What's the marker size in your case? I have consistently observed that if u load the sonicated samples directly (without phenol purifyling and having 1% SDS coming from lysis buffer in my case) onto the agarose gel, you get some weared pattern. I am sure you have done the same. So my suggesiton is that after RNase-proteinaseK incubation give phenol-chloroform treatment followed by alcohol (isopropanol or ethanol) precipitation and than load the samples on gel for analyzing the size.
rmbioMember Since 26 Jan 2009
Offline Last Active Apr 16 2013 03:51 AM
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My research interests
Molecular cloning, Gene expression, Plant volatiles