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rhombus

Member Since 26 Jan 2009
Offline Last Active Yesterday, 05:18 AM
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#116196 Splitting A549 cells

Posted biochem88 on 28 July 2011 - 05:23 AM

View Postleelee, on 27 July 2011 - 08:41 PM, said:

Just an observation, 30ml seems like a heck of a lot of media for a T75 flask for adherrent cells.

I think I have read that the volume range for a T75cm2 flask is 15-37.5ml for optimal CO2 penetration.
Also, they are going to be growing over the weekend.
It was just what I was also told to do by the person growing the cells.


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