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I always used to add antimitotics while differentiating these cells. Also RA after a while storing at -80C can degrade, so i get new ones frequently.
We use Neurobasal with B27 as the differentiating media and no FCS.
#128887 RA-differentiation of SH-SY5Y
Posted
on 10 February 2012 - 11:09 AM
#128885 Help. Lentivirus. Good titer but no transduction
Posted
on 10 February 2012 - 11:07 AM
Have you tried different concentrations of the virus?
Have a positive control for the transduction.
How long have you incubated cells with virus?
Have a positive control for the transduction.
How long have you incubated cells with virus?
#128356 troubles in DNA digestion
Posted
on 03 February 2012 - 01:14 PM
Its difficult to pipette 0.01ul of enzyme.
You need to have a positive control for the enzymes to know if they digest it properly.
there are ways to calculate DNA from agarose , by running different volumes of known conc. of ladder and comparing the intensity of your sample to the ladder.
I would suggest digesting much more DNA and digest it in 20-50ul
You need to have a positive control for the enzymes to know if they digest it properly.
there are ways to calculate DNA from agarose , by running different volumes of known conc. of ladder and comparing the intensity of your sample to the ladder.
I would suggest digesting much more DNA and digest it in 20-50ul
#96120 Alternative to Excel for charts
Posted
on 29 December 2010 - 11:15 AM
graphpad is quiet good. One can do lots of complex stuff with this.
