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- Most Active In Molecular Biology (67 posts)
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- Member Title a student
- Age 35 years old
- Birthday August 10, 1976
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In Topic: How long do growth factors last in complete media?
17 February 2012 - 10:11 AM
I used to change media with some neurotrophic factors,twice a week. So I guess they should be stable for 3-4 days.
In Topic: Problem with transwell migration
17 February 2012 - 09:59 AM
some cells are not suitable for migration. Has this cell line been described in literature for these experiments.
In Topic: No DNA after midiprep
17 February 2012 - 09:54 AM
how long do you shake the cultures? Longer shaking periods can at times lead to similar results.
Try shorter times.
Try shorter times.
In Topic: Need an effective siRNA/ shRNA sequence for downregulating caveolin-1
17 February 2012 - 09:52 AM
check sigma's site. They might have the siRNA sequence. These sequences are supposed to have been tested in their labs.
In Topic: DAB staining and GFP
15 February 2012 - 12:21 PM
In our hands, we lose the GFP fluorescence with DAB but we can stain for GFP and visualize GFP expression with DAB.
To do a costain, not need for DAB, simply go ahead with fluorescence staining.
To do a costain, not need for DAB, simply go ahead with fluorescence staining.
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