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# claritylight

Member Since 22 Mar 2005
Offline Last Active Feb 01 2013 03:23 PM

### In Topic: do I have enough sample?

01 February 2013 - 05:23 AM

Thanks bob1. That makes sense now. I can use that formula.

### In Topic: formula to convert RFU to ng/ul

01 February 2013 - 05:17 AM

I have the equation for the line. How do I now convert the RFU into the concentration now? What do I plug into the equation? Thanks.

### In Topic: do I have enough sample?

31 January 2013 - 04:48 AM

Is this correct then. I used 62ul instead of 60ul to have a little extra volume. Please correct me:

Given : 50ng/60ul = 0.833ng/ul
Sample 1 - 120ng in 42ul = 2.85ng/ul
Sample 2 - 65ng in 38ul = 1.71ng/ul
Sample 3 - 65ng in 95ul = 0.684ng/ul
Sample 4- 71ng in 76ul = 0.934ng/ul

Calculations:
sample 1 : (0.833/2.857)*62 = 18.0ul DNA + 44ul water
sample 2: (0.833/1.71)*62 = 30.2ul + 31.8ul water
sample 3: (0.833/0.684)*62ul = not enough
sample 4: (0.833/0.932)*62ul = 55.4ul + 6.6ul water

### In Topic: formula to convert RFU to ng/ul

31 January 2013 - 04:43 AM

I have a standard curve. How do I determine the ng/ul from the RFU and standard curve?