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lula

Member Since 04 Mar 2005
Offline Last Active Apr 18 2013 12:03 AM
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#145001 impurities after DNA extraction with promega kit!!!!!!

Posted bob1 on 10 November 2012 - 01:00 PM

Oh, right - Hb, I just thought you had made a typo or something.  Usually, blood extractions would be pretty clean, though it might depend on if they did a buffy coat or not, and how much blood they loaded into the extraction.

You could try to clean up the DNA by putting it through a salt precipitation and/or a phenol-chloroform extraction.


#140722 mitochondrial DNA

Posted hobglobin on 05 September 2012 - 07:57 AM

I'd say most kits are reliable (I only used a few and did not find much differences, except the price). Salting out works too for mtDNA but the DNA is more "dirty", which is for PCRs usually no problem.


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