Thanks for the reply.
Yes, it is way too high. We tried it because it says so in the book that came with the Multiphor, but I'm pretty sure it is a typo (Electrophoresis in Practice is full of typos). However, it also did not work very well with an extract with Tris 0.125M (the MW standard run well).
I have also used the paper wicks as it says in the manual.
During the run with Tris 0.125M pH 6.8 (in the stacking gel), when the bromphenol blue entered the resolving gel, two blue bands formed that were 1 cm apart. I think that that caused the loss of resolution. I think there is trouble with the ionic strenght but I cannot find any info online about running SDS-PAGE gels on flatbed systems. There is much more info about SDS gels run on the multiphor when used as the second dimension in 2D gels, but the recipes for the buffers are different.
GlyoximeMember Since 18 Feb 2005
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