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epimaster → methylnick
Dear Nick, Hello after a long time, good news thst I had a poster presentaion on Nove.13-16.2010 in San Diego, annual neuroscience conference, according thoese results which I got from epigentinc work which i had,(bisulfite treatment) :)
Jan 19 2011 09:52 PM
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methylnick
That is not true, you definitely need proteinase K to remove all the protein.
standard methods are just fine for bisulphite conversion
standard methods are just fine for bisulphite conversion
Aug 04 2010 01:51 PM
methylnick → BillyJane
Hi BillyJane, on the contrary you want to proteinaseK digest your genomic DNA to remove proteins that may interfere with the conversion. High quality DNA with a good 260/280 ratio is best for conversion. You must ensure proper denaturation of your DNA prior to conversion, 37C with 3mM NaOH is good, I tend to biol my DNA prior to adding bisulfite. cheers
nick
nick
Mar 30 2009 01:23 PM
BillyJane → methylnick
Hi MethylNick, could you give me a little hint at how to prepare DNA for Bisulfite treatment. I heard recently not to treat DNA with proteinase K cose it fragmentates the DNA..? Thax. BillyJane
Mar 30 2009 08:04 AM
