sentinel01ex

Hello!

I am using the Labtek slide chamber (Nunc) for bone marrow derived macrophages cultivation and later infected with Leishmania.
I found the cells did not adhere evenly on the surface of the wells. They were on the edge of the well, instead on the center! (I hardly found any cells in center....)
Besides, I tried to infected the macrophages with Leishmania (a free-swimming protozan parasite). The Leishmania just sticked tightly to the surface of the Labtek. And it is impossible to wash them away with simply pipetting....

So my questions are:
1. How to let the macrophages adhere to the Labtek surface evenly???
2. How to wash the wells efficiently, so that uninternalized parasites can be washed away?

Would be anyone here has similar experience to share or give me some advice?? Please please help me!

Thanks a LOT!!

poor cremebrulee