memari

I am sure but as I heard that somebody had the same problem in our university,
I think that some qPCR machines are not compatible with evagreen.

we use this machine and works well with evagreen:
http://www.illumina....s/eco-qpcr.ilmn
althogh it is not durable.

https://babakmemari....utsmart-buffer/

What is CutSmart Buffer?  Over 200 restriction enzymes are 100% active in a single buffer, CutSmart Buffer:
http://www.nebcutsmart.com
CutSmart Buffer:
50 mM Potassium Acetate
20 mM Tris-acetate
10 mM Magnesium Acetate
100 μg/ml BSA
pH 7.9

Today I did some RNA extraction and I found that if you mix Trizol or RNAzol with column base kits you have a cleaner RNA with less tendency for decreasing RNA.
RNAzol + Aurum Total RNA Mini Kit   gave me the cleanest RNA. I mean Aurum Total RNA Mini Kit gave me clean(260/280= 2.08,  260/230=2.11) RNA but after 30 minutes I had a lot of decrease of RNA. But in RNAzol + Aurum Total RNA Mini Kit, Total RNA decreased from 98 to 96 microgram, but in Aurum Total RNA Mini Kit alone I saw a decrease from 102 to 80.

I suggest you to do a RNA extraction first by RNAzol (it is much more cheaper than Trizol  www.mrcgene.com/rnazol.htm  )
, then after twice washing its RNA pelete (I suggest moving RNA pellete to new vial between these two washing steps) by 70-75 % ethanol, start using a column base kits to clean it again.

I have used RNeasy (Plus). It is completely similar to Aurum Total RNA Mini Kit but more expensive.
They start with adding 350 ul lysis buffer contanning 1 % Mercaotpethanol. Then adding 350 ul 70-75 % ethanol and passing it through  a column and then washing and eluting.

A colony origins from one  to more than 100 bacteria.
Use a inoculating turntable, and an Ultrasonic bath(Jewelry Cleaner) to disociate bateria from each other.

I call this a Obsession.