hey folks - I've recently picked up a new project, with very new techniques, and I've run into a minor stumbling block.
our collaborators have sent me some nanoparticle they have made. they have conjugated it to an antibody we use for targeting certain cell types. I need to assay for functionality to be certain our antibody targeting is not hindered by addition of the nanoparticle.
our typical plate-based cell binding assays involve adding our antibody to cells (human lymphoma and/or leukemia cells), going through a few wash steps, and assaying for remaining antibody (unbound Ab will be removed with supernatant when cells are pelleted).
well, the NP is very dense and can't be washed away. any spin that will pellet out cells will also pellet out Ab.
the NP is also magnetic, but I don't know a way to clearly pull the cells-Ab-NP complex out and quantify binding.
does anyone have any suggestions?
aimikinsMember Since 27 Aug 2004
Offline Last Active May 07 2013 01:34 PM
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I have been and done many things...currently, clinically-relevant hemoncology research. from public health, to marrow transplants in leukemic mice, to generating phage libraries, to making fusion proteins for the production of endotoxin; I'm an all-around tech.
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