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- Member Title Sunflower
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- Birthday April 2, 1976
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My research interests
I have been and done many things...currently, clinically-relevant hemoncology research. from public health, to marrow transplants in leukemic mice, to generating phage libraries, to making fusion proteins for the production of endotoxin; I'm an all-around tech.
#154056 2D Image Primary Cell line
Posted
mdfenko
on 22 April 2013 - 04:40 AM
you can download a useful handbook on 2d electrophoresis (as well as other useful handbooks) from ge healthcare life sciences.
#153741 New to the field - literature advice needed
Posted
hobglobin
on 15 April 2013 - 01:39 AM
I'd say most important for plants are mycorrhiza fungi which you should not forget. You can detect them even with simple microscopy and suitable staining methods. Even quantification is possible with quite easy methods (e.g. percentage of roots with mycorrhiza).
Anyway your approach is quite challenging esp for a beginner as you work with an extreme complex and dynamic system which you have no idea when (or only when it's too late), how and why it changes. And you cannot control changes in terms quality and quantity and possible direction of changes, as you have no measures except the proper soil smell.
A more scientific approach would be to work with a standardisation (only one soil type with defined characteristics as I write below) and simplification (i.e. one or few MOs) so that you can tell which changes cause which results and then go to more complex experiments.
Don't forget that also other factors such as humidity, soil texture, pH, nutrient content and organic matter content highly influence plants, MOs and all other species in soil and that these factors also influence each other to a high degree (e.g. influences the pH value the availability of nutrients). You should consider to measure these values as far as possible (humidity regime, pH measurements).
For pH and nutrient measurements kits for gardeners are available, that you can get an idea about this and they are quite cheap.
For inoculation of soils with beneficial MOs also ready-made cultures are available that should support the fertility of soil. Esp. many organic gardeners use them to avoid synthetic fertilisers. You should check this (also to avoid double work
).
Anyway your approach is quite challenging esp for a beginner as you work with an extreme complex and dynamic system which you have no idea when (or only when it's too late), how and why it changes. And you cannot control changes in terms quality and quantity and possible direction of changes, as you have no measures except the proper soil smell.
A more scientific approach would be to work with a standardisation (only one soil type with defined characteristics as I write below) and simplification (i.e. one or few MOs) so that you can tell which changes cause which results and then go to more complex experiments.
Don't forget that also other factors such as humidity, soil texture, pH, nutrient content and organic matter content highly influence plants, MOs and all other species in soil and that these factors also influence each other to a high degree (e.g. influences the pH value the availability of nutrients). You should consider to measure these values as far as possible (humidity regime, pH measurements).
For pH and nutrient measurements kits for gardeners are available, that you can get an idea about this and they are quite cheap.
For inoculation of soils with beneficial MOs also ready-made cultures are available that should support the fertility of soil. Esp. many organic gardeners use them to avoid synthetic fertilisers. You should check this (also to avoid double work
#152659 Same first name and second name of two authors in one paper???
Posted
pito
on 21 March 2013 - 09:37 AM
mansi368, on 21 March 2013 - 09:28 AM, said:
Hi..
This is heights of coincidence but there are two people of same first name and second name in my lab. Both of them worked equally for one publication and morally both own it..
The problem is how to write their names as both first name and second name are same??? The computer everytime takes it as an anomaly and erases the name written twice......
What to do please suggest
Thanks
This is heights of coincidence but there are two people of same first name and second name in my lab. Both of them worked equally for one publication and morally both own it..
The problem is how to write their names as both first name and second name are same??? The computer everytime takes it as an anomaly and erases the name written twice......
What to do please suggest
Thanks
Or dont put them next to eachother?
Or use a sign next to their name that means: contributed equally for example *1 next to the first name and *2 next to the second
#151667 difference between monoclonal antibody and polyclonal antibody?
Posted
doxorubicin
on 06 March 2013 - 09:54 AM
I agree with Tabaluaga's definition. Also, you can think of an antibody as a protein that has a specific amino acid sequence. A monoclonal antibody solution that you purchase should consist only of identical antibodies, all with the exact same amino acid sequence. A single B cell containing just the right DNA sequence was isolated, immortilized, and amplified to give a population of cells that all make precisely that one antibody. Polyclonal antibodies are generally purified directly from serum, so they're the combined efforts of many B cells, each of which might have produced a distinct sequence of amino acids that happens to have affinity for the immunogen.
#151657 difference between monoclonal antibody and polyclonal antibody?
Posted
Tabaluga
on 06 March 2013 - 08:54 AM
A monoclonal antibody is raised against a specific single epitope of a certain antigen. It is called monoclonal because it is derived from a single clone of identical immune cells which are derived from a unique parent cell. Polyclonal antibody is a mixture of antibodies from different immune cells that are raised against the same antigen, but recognize different epitopes. They are not derived from a single clone of immune cells.
#151620 virus and cell surface receptor interaction
Posted
bob1
on 05 March 2013 - 07:37 PM
The only thing I would add is that the virus uncoats inside the cell - otherwise the stuff inside the virus will not be replicated...
#151715 how to determine the serotype of a pathogen?
Posted
bob1
on 06 March 2013 - 04:47 PM
Essentially the serotype of a disease is a variation in the cell surface proteins which cause immunological reactions in the infected person. You can group different subspecies of organism together based on the serotype.
For a good clear example, look up Lancefield grouping.
If an organism causes infection in both humans and other animals, then of course there is potential for cross infection, but this has nothing to do with the serovar.
For a good clear example, look up Lancefield grouping.
If an organism causes infection in both humans and other animals, then of course there is potential for cross infection, but this has nothing to do with the serovar.
#151678 how to determine the serotype of a pathogen?
Posted
bob1
on 06 March 2013 - 11:52 AM
Look at a range of serums from people with the disease and see how many of them cross-react with different strains of the disease...
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