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- Group Global Moderators
- Active Posts 115
- Profile Views 5,923
- Member Title Sunflower
- Age 37 years old
- Birthday April 2, 1976
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Gender
Not Telling
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Location
Seattle, WA
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Hobbies
www.fhcrc.org
About me
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My research interests
I have been and done many things...currently, clinically-relevant hemoncology research. from public health, to marrow transplants in leukemic mice, to generating phage libraries, to making fusion proteins for the production of endotoxin; I'm an all-around tech.
Posts I've Made
In Topic: competent cells
22 May 2013 - 12:52 PM
if you make home-grown competent cells, there are a number of Cl salts. the Cl salts in the preparation help open up channels in the membrane.
In Topic: Cloning advice
22 May 2013 - 09:00 AM
sometimes you lose things during cloning. typically there is something unanticipated like a recombination event or poor primer design.
do you have other colonies you picked from the cloning to sequence?
do you have other colonies you picked from the cloning to sequence?
In Topic: Cloning advice
21 May 2013 - 01:41 PM
have you repeated the sequencing in both directions and verified the result?
In Topic: Luminescence: Background or real signal?
26 April 2013 - 11:57 AM
hmm.... as far as your low values, if they are below your "blank" or negative control values, you need a different negative control. can you list in detail what you have tried?
In Topic: New to the field - literature advice needed
13 April 2013 - 01:30 PM
these are very open-ended questions, and tough to answer. it would help if we understood more specifically what it is you are trying to accomplish.
what do you mean by "quality"?
as far as methods of culturing, what are you trying to enrich for?
what do you mean by "store"? are you talking about keeping for weeks, months, years?
what do you mean by "quality"?
as far as methods of culturing, what are you trying to enrich for?
what do you mean by "store"? are you talking about keeping for weeks, months, years?
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