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Hi
I am performing luciferase assay with 2 expression vectors+ 1 promoter-luciferase vector+GFP- control vector.
So each time i perform the experiment I get extremely different readings.
Could it be because one of the vectors is not getting transfected, even though i get a pretty decent transfection efficiency. Also, can only the GFP vector be transfected and not the others?
Suggestions and ideas would be immensely helpful
